Journal of Food Bioactives, ISSN 2637-8752 print, 2637-8779 online
Journal website www.isnff-jfb.com

Original Research

Volume 25, March 2024, pages 62-71

A novel combination against skin aging via promoting the synthesis of biological collagen

Figures

Figure 1.
Figure 1. The effect of Gancao, Baizhi, Baihe, and Dazao extracts on cell viability and the mRNA levels of collagen. (a) Determination of cell viability in NIH-3T3 cells pretreated with ten CHM extracts. The mRNA levels of collagen I (b) and collagen III (c) in NIH-3T3 cells.
Figure 2.
Figure 2. The activity and stability of P4H. (a) MALDI-TOF-TOF mass spectra of (PAGP)3 before and after application of P4H. (b) Effect of different temperatures on P4H stability.
Figure 3.
Figure 3. The P4H with homogeneous particle size and good transdermal rate was successfully prepared. (a) Transmission electron microscopy image of the P4H transfersome. (b) Cumulative P4H permeation of P4H transfersome solution, P4H liposome solution, and P4H solution, respectively.
Figure 4.
Figure 4. The combinations improve the content of hydroxyproline in NIH-3T3 cells.
Figure 5.
Figure 5. HE staining of cross-sectional slices from the dorsal skin of mice, magnification, ×100.
Figure 6.
Figure 6. The hydroxyproline content in skin tissue of mice.

Tables

Table 1. Primer list used in this study
 
PrimerSequence
GAPDHF: TTGCTGATCCACATCTGCTGGAAG
R: GGCCACTGCCGCATCCTCTTC
Collagen-IF: ATGTTCAGCTTTGTGGACCTCCGGC
R: CTTAGGACCAGCAGGACCAGTATCT
Collagen-IIIF: ATGATGAGCTTTGTGCAATGTGGGA
R: CATGGGGCCAGGAGCTCCGTTGTCT
pET16b(+)F: GCTCTAGAATGGAGGGGTTTGAAACCAGCGAT
R: CATGCCATGGTTTAACAGCACGGATCCATC

 

Table 2. Factors and Levels
 
LevelA (w/w)B (%)C (mg/mL)
A, B and C are the ratio of soy lecithin and cholesterol, total lipid concentration, and enzyme concentration, respectively.
190 : 1062
285 : 1535
380 : 20110

 

Table 3. The components of different combinations
 
CombinationComponents
CHM-1-1Dazao + P4H transfersome + VC
CHM-1-2Gancao + P4H transfersome + VC
CHM-1-3Baizhi+ P4H transfersome + VC
CHM-1-4Baihe + P4H transfersome + VC
CHM-2-1Dazao + Gancao + P4H transfersome + VC
CHM-2-2Dazao + Baizhi + P4H transfersome + VC
CHM-2-3Dazao + Baihe + P4H transfersome + VC
CHM-2-4Gancao + Baizhi + P4H transfersome + VC
CHM-2-5Gancao + Baihe + P4H transfersome + VC
CHM-2-6Baihe + Baizhi + P4H transfersome + VC
CHM-3-1Dazao + Gancao + Baizhi+ P4H transfersome + VC
CHM-3-2Dazao + Gancao + Baihe P4H transfersome + VC
CHM-3-3Dazao + Baizhi + Baihe+ P4H transfersome + VC
CHM-3-4Baizhi + Baihe+ Gancao + P4H transfersome + VC
CHM-4-1Dazao + Baizhi + Baihe+ Gancao + P4H transfersome + VC

 

Table 4. The experimental animial grouping
 
Grouping0.9% saline water (mL/d)10% D-gal injection (mL/d)Dose of application (mL/d)
Control0.200
Model00.20
L (200 mg/mL CHM-3-4 )00.20.2
M (400 mg/mL CHM-3-4 )00.20.2
H (800 mg/mL CHM-3-4 )00.20.2

 

Table 5. Encapsulation efficiency of P4H at different factor levels of the ratio of soy lecithin and cholesterol (A), total lipid concentration (B), and drug concentration (C), respectively
 
NumberABCEncapsulation efficiency(%)
112122.0
211236.9
313318.5
422215.6
521326.4
623113.6
732324.5
831120.9
933222.1

 

Table 6. Intuitive analysis of the test results
 
IndexABC
K175.484.254.5
K255.660.174.6
K367.554.269.4
k125.128.118.2
k218.520.024.9
k322.518.123.1
R6.610.06.7