| Journal of Food Bioactives, ISSN 2637-8752 print, 2637-8779 online |
| Journal website www.isnff-jfb.com |
Review
Volume 24, December 2023, pages 40-45
Research progress on separation and characterization of bioflavonoids from Coreopsis tinctoria Nutt.
Table
| Purification methods | Advantages | Disadvantages |
|---|---|---|
| Macroporous adsorption resin method | Good yield and purity; simple equipment and easy operation; multiple types of adsorbents, wide range of applications; reusable/regenerable resin, long service life; overall low cost, suitable for industrial production | Bad desorption effect; time consuming; separation affected by adsorbent performance, eluent type, temperature, etc. |
| Polyamide column chromatography | Good separation effect and high product purity; good specificity; large sample capacity; reusable/regenerable resin, overall low cost, suitable for industrial production | Long separation period; large void adsorption volume; co-elution with low molecular weight impurities; adsorption process readily affected by solvents |
| Silica gel column chromatography | Large adsorption capacity and wide application range; easy separation effect; broad use; inexpensive material | Low separation efficiency; disposable material, organic solvent elution |
| Sephadex gel chromatography | Wide range of use; good separation efficiency; reusable/regenerable resin | Elution influenced by the number of phenolic hydroxyl groups and size and molecular weight of molecules; expensive materials |
| High-speed counter current chromatography or high-performance counter current chromatography | High purity of product; good reproducibility; separation of multiple components; automation | High cost; low productivity; low separation capacity; often time consuming |
| Reversed-phase high performance liquid chromatography | Good separation; multiple component separation capacity; automation | Very high cost equipment; low capacity; hard to meet animal study |