Journal of Food Bioactives, ISSN 2637-8752 print, 2637-8779 online
Journal website www.isnff-jfb.com

Original Research

Volume 22, June 2023, pages 43-52

Protective effect of Melanogrammus aeglefinus skin oligopeptide in ultraviolet B-irradiated human keratinocytes

Figures

Figure 1.
Figure 1. Interactions between Keap1 and peptide predicated Discovery Studio 2019. (a) VADML, (b) IARF, (c) SSPSF.
Figure 2.
Figure 2. Antioxidant activity of MSOP in human keratinocytes HaCaT cells exposed to UVB. (a) Cell viability in response to UVB and MSOP treatments. (b) activity of SOD. (c) Content of MDA. (d) Activity of GSH-Px. ### p < 0.001 compared to control group. *p < 0.05, **p < 0.01 and ***p < 0.001 compared to UVB group.
Figure 3.
Figure 3. The anti-apoptosis effects of MSOP measured by flow cytometric analysis. (a) Control group. (b) UVB group. (d) High-dosage UVB group.
Figure 4.
Figure 4. Quantitative proteome analysis in HaCaT cells in response to UVB and MSOP treatments. (a) Venn diagram of differentially expressed proteins (DEPs) (fold change ≥2 or ≤0.5) in response to UVB and MSOP treatments. (b) Numbers of upregulated and downregulated proteins in HaCaT cells. Upregulated (c) and downregulated (d) DEPs categorized by biological process, molecular function, and cellular component.
Figure 5.
Figure 5. Expression of differentially expressed proteins measured by quantitative proteome and qRT-PCR in HaCaT cells. (a) qcr2, (b) calX, (c) uba1, (d) dhe3, (e) lap2B, (f) ehd2, (g) nrf2, (g) 6pgd, (i) serA, (j) keap1, (k) lamP1, (l) ero1A.
Figure 6.
Figure 6. General scheme for the beneficial effects of MSOP through Keap1/Nrf2/ARE signaling pathway regulation.

Tables

Table 1. The potential targets of three peptides with high fit value screened by Discovery Studio 2019
 
PeptidePharmacophoreFit valueBiological function
VADML4zy3-04: Kelch-like ECH-associated protein 13.9737Antioxidation
1pg4-06: Acetyl-coenzyme A synthetase3.8309Biosynthesis of antibiotics
1uk4-02: Replicase polyprotein 1ab3.81262Severe acute respiratory syndrome
IARF5dad-08: Kelch-like ECH-associated protein 13.78306Antioxidation
1a4h-07: ATP-dependent molecular chaperone HSP823.73784cancer
SSPSF1tyr-01: Transthyretin3.92604Hyperthyroxinemia,
1tqf-02: Beta-secretase 13.91493Alzheimer’s disease
4zy3-03: Kelch-like ECH-associated protein 13.85125Antioxidation
1m48-04: Interleukin-23.77783Type I diabetes mellitus
3cqw-09: RAC-alpha serine/threonine-protein kinase3.75172cancer

 

Table 2. The molecular docking results of three peptides with Keap1
 
PeptideAmino Acid Sequence-ECI (kJ/mol)-EVDW (kJ/mol)RMS Gradient
VADMLVal-Ala-Asp-Met-Leu92.268510.05150.00935
IARFIle-Ala-Arg-Phe73.59675.614570.00984
SSPSFSer-Ser-Pro-Ser-Phe63.02323.696580.00985