J Food Bioact

Journal of Food Bioactives, ISSN 2637-8752 print, 2637-8779 online

Journal website www.isnff-jfb.com

Original Research

Volume 7, September 2019, pages 56-62

Modulatory effect of selected phenolic acids on enzyme activities of purinergic signaling

Figure 1. Effect of the phenolic acids (caffeic, ferulic, chlorogenic and p-coumaric acid) on Na⁺/K⁺-ATPase Activity in isolated rat brain. Values represent mean ± SD of triplicate experimental readings.

Figure 2. Effect of the phenolic acids (caffeic, ferulic, chlorogenic and p-coumaric acid) on ATPDase activity in isolated rat brain. Values represent mean ± SD of triplicate experimental readings.

Figure 3. Effect of the phenolic acids (caffeic, ferulic, chlorogenic and p-coumaric acid) on ecto-5′ nucleotidase activity in isolated rat brain. Values represent mean ± SD of triplicate experimental readings.

Figure 4. Effect of the phenolic acids (caffeic, ferulic, chlorogenic and p-coumaric acid) on phosphodiesterase-5-activity in isolated rat brain. Values represent mean ± SD of triplicate experimental reading.

Figure 5. Inhibition of Fe²⁺ induced TBARS Production in rat’s brain by phenolic acids (caffeic, ferulic, chlorogenic and p-coumaric acid). Values represent mean ± SD of triplicate experimental reading.

**Table 1.** IC₅₀ values (µM) for the inhibitory effect of selected phenolic acids (caffeic, ferulic, chlorogenic and p-coumaric acids) on Na⁺/K⁺-ATPase, ATPdase, Ecto-5′nucleotidase and phosphodiesterase-5′activities in isolated rat brain homogenates
Enzyme activity	Caffeic Acid	Ferulic Acid	Chlorogenic Acid	p-coumaric Acid
Values represent means ± standard deviation (n = 3). ^a–dMean values with the same superscript letter on the same row are not significantly different (p > 0.05).
Na⁺/K⁺-ATPase	75.57 ± 0.22^c	3.48 ± 0.09^a	253.9 ± 0.14^d	48.59 ± 0.15^b
ATPDase	346.9 ± 0.03^d	27.48 ± 0.02^c	18.37 ± 0.07^b	17.56 ± 0.04^a
Ecto-5′ nucleotidase	8.25 ± 4.13^a	6.22 ± 1.76^a,b	12.48 ± 3.26^b	35.31 ± 1.59^c
Phosphodiesterase-5′	35.73 ± 0.11^b	400.3 ± 0.05^d	21.03 ± 0.21^a	128.4 ± 0.08^c

Table 1. IC₅₀ values (µM) for the inhibitory effect of selected phenolic acids (caffeic, ferulic, chlorogenic and p-coumaric acids) on Na⁺/K⁺-ATPase, ATPdase, Ecto-5′nucleotidase and phosphodiesterase-5′activities in isolated rat brain homogenates

Enzyme activity

Caffeic Acid

Ferulic Acid

Chlorogenic Acid

p-coumaric Acid

Values represent means ± standard deviation (n = 3). ^a–dMean values with the same superscript letter on the same row are not significantly different (p > 0.05).

Na⁺/K⁺-ATPase

75.57 ± 0.22^c

3.48 ± 0.09^a

253.9 ± 0.14^d

48.59 ± 0.15^b

ATPDase

346.9 ± 0.03^d

27.48 ± 0.02^c

18.37 ± 0.07^b

17.56 ± 0.04^a

Ecto-5′ nucleotidase

8.25 ± 4.13^a

6.22 ± 1.76^a,b

12.48 ± 3.26^b

35.31 ± 1.59^c

Phosphodiesterase-5′

35.73 ± 0.11^b

400.3 ± 0.05^d

21.03 ± 0.21^a

128.4 ± 0.08^c

**Table 2.** IC₅₀ values (µM) on the inhibitory effect of selected phenolic acids (caffeic, ferulic, chlorogenic and p-coumaric acids) on Fe²⁺-induced TBARS production in isolated rat brain homogenates as well as their radical scavenging ability against DPPH and ABTS radicals
	Caffeic Acid	Ferulic Acid	Chlorogenic Acid	p-coumaric Acid
Values represent means ± standard deviation (n = 3). ^a–dMean values with the same superscript letter on the same row are not significantly different (p > 0.05). *µmol. Trolox Antioxidant Equivalent.
TBAS Production	14.85 ± 0.12^b	81.38 ± 0.04^d	7.54 ± 6.42^a	32.61 ± 2.87^c
DPPH scavenging ability	91.73 ± 1.70^d	79.42 ± 1.59^c	10.33 ± 8.89^a	20.30 ± 3.01^b
ABTS scavenging ability*	8.72 ± 0.07^b	13.88 ± 0.06^c	7.211 ± 0.06^a	63.07 ± 0.06^d

Table 2. IC₅₀ values (µM) on the inhibitory effect of selected phenolic acids (caffeic, ferulic, chlorogenic and p-coumaric acids) on Fe²⁺-induced TBARS production in isolated rat brain homogenates as well as their radical scavenging ability against DPPH and ABTS radicals

Caffeic Acid

Ferulic Acid

Chlorogenic Acid

p-coumaric Acid

Values represent means ± standard deviation (n = 3). ^a–dMean values with the same superscript letter on the same row are not significantly different (p > 0.05). *µmol. Trolox Antioxidant Equivalent.

TBAS Production

14.85 ± 0.12^b

81.38 ± 0.04^d

7.54 ± 6.42^a

32.61 ± 2.87^c

DPPH scavenging ability

91.73 ± 1.70^d

79.42 ± 1.59^c

10.33 ± 8.89^a

20.30 ± 3.01^b

ABTS scavenging ability*

8.72 ± 0.07^b

13.88 ± 0.06^c

7.211 ± 0.06^a

63.07 ± 0.06^d