J Food Bioact

Journal of Food Bioactives, ISSN 2637-8752 print, 2637-8779 online

Journal website www.isnff-jfb.com

Original Research

Volume 3, Number , September 2018, pages 133-143

Antioxidant and antihypertensive activities of wonderful cola (Buchholzia coriacea) seed protein and enzymatic protein hydrolysates

Figure 1.

Free radical scavenging activity of glutathione (GSH), wonderful cola protein isolate (WCI), hydrolysate (WCH) and ultrafiltration fractions. (a) DPPH; (b) Hydroxyl.

Figure 2.
(a) Ferric reducing antioxidant power and (b) Metal chelation activity of glutathione (GSH), wonderful cola protein isolate (WCI), hydrolysate (WCH) and ultrafiltration fractions.

Figure 3.
(a) Superoxide scavenging and (b) ORAC activities of glutathione (GSH), wonderful cola protein isolate (WCI), hydrolysate (WCH) and ultrafiltration fractions.

Figure 4.
Inhibition of in vitro activities of ACE (a) and renin (b) by wonderful cola protein isolate (WCI), hydrolysate (WCH) and ultrafiltration fractions.

Figure 5.

Antihypertesnive effects of wonderful cola protein isolate (WCI), hydrolysate (WCH) and ultrafiltration fractions after oral administration to spontaneously hypertensive rats. (a) Systolic blood pressure (SBP); (b) Diastolic blood pressure (DBP).

**Table 1.** Amino acid profile (g/100 g protein) of wonderful cola protein isolate (WCI), hydrolysate (WCH) and ultrafiltration fractions
Amino acids	WCI	WCH	Fractionated peptides
Thr	3.86 ± 0.07	3.76 ± 0.02	3.54 ± 0.13	3.90 ± 0.03	3.93 ± 0.06	4.11 ± 0.11
Val	4.52 ± 0.06	4.79 ± 0.19	5.23 ± 0.10	5.35 ± 0.19	5.34 ± 0.08	5.39 ± 0.10
Met	1.18 ± 0.10	1.19 ± 0.07	0.72 ± 0.01	0.82 ± 0.01	0.83 ± 0.05	0.79 ± 0.00
Ile	3.96 ± 0.02	3.64 ± 0.03	3.96 ± 0.03	3.81 ± 0.02	4.54 ± 0.26	4.46 ± 0.20
Leu	8.61 ± 0.10	7.68 ± 0.19	10.06 ± 0.05	8.67 ± 0.28	8.32 ± 0.17	7.41 ± 0.34
Phe	7.14 ± 0.02	6.46 ± 0.01	10.59 ± 0.39	8.45 ± 0.35	7.56 ± 0.04	5.82 ± 0.05
His	4.53 ± 0.44	3.63 ± 0.30	3.66 ± 0.27	3.63 ± 0.25	3.54 ± 0.01	3.93 ± 0.02
Lys	4.49 ± 0.05	4.50 ± 0.39	3.79 ± 0.00	3.93 ± 0.05	3.98 ± 0.02	4.64 ± 0.02
Trp	1.65 ± 0.10	1.51 ± 0.22	1.59 ± 0.03	1.90 ± 0.08	1.81 ± 0.03	1.64 ± 0.01
Arg	8.43 ± 0.08	9.10 ± 0.01	10.74 ± 0.03	8.75 ± 0.02	7.46 ± 0.37	7.03 ± 0.03
Tyr	5.62 ± 0.01	5.33 ± 0.27	8.43 ± 0.20	6.72 ± 0.20	6.53 ± 0.34	4.33 ± 0.08
Ser	5.12 ± 0.10	4.92 ± 0.05	4.11 ± 0.09	4.67 ± 0.11	4.71 ± 0.13	4.99 ± 0.00
Glu	13.93 ± 0.15	17.04 ± 0.16	13.12 ± 0.21	13.89 ± 0.17	14.52 ± 0.09	14.70 ± 0.04
Pro	6.76 ± 0.08	6.62 ± 0.32	3.39 ± 0.05	5.58 ± 0.07	6.43 ± 0.28	7.67 ± 0.18
Gly	4.59 ± 0.34	5.40 ± 0.15	4.33 ± 0.27	5.59 ± 0.39	5.42 ± 0.20	6.70 ± 0.30
Asp	11.26 ± 0.15	10.21 ± 0.19	7.61 ± 0.23	9.81 ± 0.12	10.58 ± 0.29	11.69 ± 0.12
Ala	4.34 ± 0.20	4.22 ± 0.19	5.13 ± 0.07	4.53 ± 0.38	4.50 ± 0.05	4.69 ± 0.20
Cys	1.97 ± 0.02	1.84 ± 0.05	1.07 ± 0.05	1.31 ± 0.08	1.45 ± 0.22	1.59 ± 0.01

Table 1. Amino acid profile (g/100 g protein) of wonderful cola protein isolate (WCI), hydrolysate (WCH) and ultrafiltration fractions

Amino acids

WCI

WCH

Fractionated peptides

<1 kDa

1–3 kDa

3–5 kDa

5–10 kDa

Thr

3.86 ± 0.07

3.76 ± 0.02

3.54 ± 0.13

3.90 ± 0.03

3.93 ± 0.06

4.11 ± 0.11

Val

4.52 ± 0.06

4.79 ± 0.19

5.23 ± 0.10

5.35 ± 0.19

5.34 ± 0.08

5.39 ± 0.10

Met

1.18 ± 0.10

1.19 ± 0.07

0.72 ± 0.01

0.82 ± 0.01

0.83 ± 0.05

0.79 ± 0.00

Ile

3.96 ± 0.02

3.64 ± 0.03

3.96 ± 0.03

3.81 ± 0.02

4.54 ± 0.26

4.46 ± 0.20

Leu

8.61 ± 0.10

7.68 ± 0.19

10.06 ± 0.05

8.67 ± 0.28

8.32 ± 0.17

7.41 ± 0.34

Phe

7.14 ± 0.02

6.46 ± 0.01

10.59 ± 0.39

8.45 ± 0.35

7.56 ± 0.04

5.82 ± 0.05

His

4.53 ± 0.44

3.63 ± 0.30

3.66 ± 0.27

3.63 ± 0.25

3.54 ± 0.01

3.93 ± 0.02

Lys

4.49 ± 0.05

4.50 ± 0.39

3.79 ± 0.00

3.93 ± 0.05

3.98 ± 0.02

4.64 ± 0.02

Trp

1.65 ± 0.10

1.51 ± 0.22

1.59 ± 0.03

1.90 ± 0.08

1.81 ± 0.03

1.64 ± 0.01

Arg

8.43 ± 0.08

9.10 ± 0.01

10.74 ± 0.03

8.75 ± 0.02

7.46 ± 0.37

7.03 ± 0.03

Tyr

5.62 ± 0.01

5.33 ± 0.27

8.43 ± 0.20

6.72 ± 0.20

6.53 ± 0.34

4.33 ± 0.08

Ser

5.12 ± 0.10

4.92 ± 0.05

4.11 ± 0.09

4.67 ± 0.11

4.71 ± 0.13

4.99 ± 0.00

Glu

13.93 ± 0.15

17.04 ± 0.16

13.12 ± 0.21

13.89 ± 0.17

14.52 ± 0.09

14.70 ± 0.04

Pro

6.76 ± 0.08

6.62 ± 0.32

3.39 ± 0.05

5.58 ± 0.07

6.43 ± 0.28

7.67 ± 0.18

Gly

4.59 ± 0.34

5.40 ± 0.15

4.33 ± 0.27

5.59 ± 0.39

5.42 ± 0.20

6.70 ± 0.30

Asp

11.26 ± 0.15

10.21 ± 0.19

7.61 ± 0.23

9.81 ± 0.12

10.58 ± 0.29

11.69 ± 0.12

Ala

4.34 ± 0.20

4.22 ± 0.19

5.13 ± 0.07

4.53 ± 0.38

4.50 ± 0.05

4.69 ± 0.20

Cys

1.97 ± 0.02

1.84 ± 0.05

1.07 ± 0.05

1.31 ± 0.08

1.45 ± 0.22

1.59 ± 0.01