Journal of Food Bioactives, ISSN 2637-8752 print, 2637-8779 online
Journal website www.isnff-jfb.com

Original Research

Volume 2, Number , June 2018, pages 119-128

Wheat gliadin deamidated by cation-exchange resins induces oral tolerance in a mouse model of wheat allergy

Figures

Figure 1.
Figure 1.

Experimental design for evaluation of induction of oral tolerance by deamidated gliadin.

Figure 2.
Figure 2.

Intestinal permeability (a) and serum histamine level (b) of gliadin-sensitized mice after repeated sample administration and gliadin challenge.

Each value is the mean of 7 experiments ± S.E. Values with an asterisk in the left figure are significantly different from those of unsensitized mice at p < 0.05 by Tukey-Kramer test. Values with different letters in the right figure are significantly different at p < 0.01 by Tukey-Kramer test.
Figure 3.
Figure 3.

Serum gliadin-specific IgE level of gliadin-sensitized mice after repeated sample administration and gliadin challenge.

Each value is the mean of 7 experiments ± S.E. Values with different letters are significantly different at p < 0.01 by Tukey-Kramer test.
Figure 4.
Figure 4.

Serum gliadin-specific IgG (a), IgG1 (b), IgG2a (c) and IgA (d) levels of gliadin-sensitized mice after repeated sample administration and gliadin challenge.

Each value is the mean of 7 experiments ± S.E. Values with different letters are significantly different at p < 0.01 by Tukey-Kramer test.
Figure 5.
Figure 5.

Serum IL-4 (a), IFN-γ (b), IL-12 (c), and IL-17 (d) levels of gliadin-sensitized mice after repeated sample administration and gliadin challenge.

Each value is the mean of 7 experiments ± S.E. Values with different letters are significantly different at p < 0.01 by Tukey-Kramer test.
Figure 6.
Figure 6.

Serum IL-10 (a) and TGF-β (b) levels of gliadin-sensitized mice after repeated sample administration and gliadin challenge.

Each value is the mean of 7 experiments ± S.E. Values with different letters are significantly different at p < 0.01 by Tukey-Kramer test.
Figure 7.
Figure 7.

Flow cytometric dot plots of Treg (CD4+FoxP3+) cells of Unsesitized (a), Control (b), UG (c), and DG (d) groups.

Figure 8.
Figure 8.

Percentage of lymphocyte cells expressing CD4+CD25+ (a) and CD4+FoxP3+ (b).

Each value is the mean of 7 experiments ± S.E. Values with different letters are significantly different at p < 0.01 by Tukey-Kramer test.
Figure 9.
Figure 9.

Schematic illustration of role of Treg cells.